Ric-8 regulation of heterotrimeric G proteins
نویسندگان
چکیده
منابع مشابه
Molecular chaperoning function of Ric-8 is to fold nascent heterotrimeric G protein α subunits.
We have shown that resistance to inhibitors of cholinesterase 8 (Ric-8) proteins regulate an early step of heterotrimeric G protein α (Gα) subunit biosynthesis. Here, mammalian and plant cell-free translation systems were used to study Ric-8A action during Gα subunit translation and protein folding. Gα translation rates and overall produced protein amounts were equivalent in mock and Ric-8A-imm...
متن کاملRegulation of Golgi Structure through Heterotrimeric G Proteins
We have previously shown that ilimaquinone (IQ), a marine sponge metabolite, causes complete vesiculation of the Golgi stacks. By reconstituting the IQ-mediated vesiculation of the Golgi apparatus in permeabilized cells, we now demonstrate that this process does not require ARF and coatomers, which are necessary for the formation of Golgi-derived COPI vesicles. We find that IQ-mediated Golgi ve...
متن کاملFunction and Regulation of Heterotrimeric G Proteins during Chemotaxis
Chemotaxis, or directional movement towards an extracellular gradient of chemicals, is necessary for processes as diverse as finding nutrients, the immune response, metastasis and wound healing. Activation of G-protein coupled receptors (GPCRs) is at the very base of the chemotactic signaling pathway. Chemotaxis starts with binding of the chemoattractant to GPCRs at the cell-surface, which fina...
متن کاملPurification of heterotrimeric G protein alpha subunits by GST-Ric-8 association: primary characterization of purified G alpha(olf).
Ric-8A and Ric-8B are nonreceptor G protein guanine nucleotide exchange factors that collectively bind the four subfamilies of G protein α subunits. Co-expression of Gα subunits with Ric-8A or Ric-8B in HEK293 cells or insect cells greatly promoted Gα protein expression. We exploited these characteristics of Ric-8 proteins to develop a simplified method for recombinant G protein α subunit purif...
متن کاملDynamic Motions of the Alpha Subunit of Heterotrimeric G Proteins in the Nucleotide- and Ric-8a-bound States
The conformational changes and segmental dynamics involved in the -subunit of heterotrimeric G proteins have been investigated for three separate binding states by using binning-timedependent single-molecule Förster resonance energy transfer (smFRET) of freely diffusing proteins and time-resolved fluorescence anisotropy. The bound states include Ric-8A (Resistance to Inhibitors of Cholinestera...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Receptors and Signal Transduction
سال: 2013
ISSN: 1079-9893,1532-4281
DOI: 10.3109/10799893.2013.763828